ELISA test for rabies antibody titration in orally vaccinated foxes sampled in the fields
Identifieur interne : 000D27 ( Main/Exploration ); précédent : 000D26; suivant : 000D28ELISA test for rabies antibody titration in orally vaccinated foxes sampled in the fields
Auteurs : F. Cliquet [France] ; L. Sagné [France] ; J. L Schereffer [France] ; M. F. A Aubert [France]Source :
- Vaccine [ 0264-410X ] ; 2000.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Rage, Vaccination.
English descriptors
- KwdEn :
- Administration, Oral, Animals, Antibodies, Viral (blood), Antibody, ELISA assay, Enzyme immunoassay, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Foxes (immunology), Neutralization Tests, Rabies, Rabies Vaccines (immunology), Rabies virus, Sanitary surveillance, Serology, Vaccination, Vaccines, Attenuated (immunology), Vaccines, Synthetic (immunology), Vulpes vulpes, Wild animal.
- MESH :
- chemical , blood : Antibodies, Viral.
- immunology : Foxes, Rabies Vaccines, Vaccines, Attenuated, Vaccines, Synthetic.
- Administration, Oral, Animals, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Neutralization Tests, Vaccination.
Abstract
The assessment of the efficacy of rabies oral vaccination campaigns requires the titration of specific antibodies in the target species. Unfortunately, in Continental Europe, most fox serum samples are in fact “body fluids” taken from cadavers and the lack of a validated titration method for these poor quality sera made it impossible to survey and compare the efficacy of various oral vaccination protocols used by the different European teams. By using ready to use microplates sensitised with rabies virus glycoprotein purchased from a manufacturer and applying a simple and rapid ELISA technique on field fox sera, we obtained antibody quantitation highly correlated with seroneutralising antibody titres measured with a seroneutralisation test on cell culture. We obtained, with fox sera sampled in the same area, the same distribution of high, medium and low titres within all categories of serum quality (from high to very poor quality) and therefore conclude that this ELISA test allows a reliable titration even with highly contaminated body fluids. This test was shown to be equally capable of detecting rabies antibodies in serum samples taken from foxes vaccinated with an highly attenuated rabies virus (the SAG2 double mutant of the Street Alabama Dufferin strain) or with the VRG, the Vaccinia recombinant glycoprotein. Additionally, a strong correlation was demonstrated between titres given by this ELISA (or by the seroneutralisation test) and protection against challenge of foxes orally vaccinated with SAG2 vaccine baits. In view of this validation, this simple and reliable test is proposed for sero-surveying foxes following rabies oral vaccination campaigns.
Url:
DOI: 10.1016/S0264-410X(00)00127-4
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000C21
- to stream Istex, to step Curation: 000C20
- to stream Istex, to step Checkpoint: 000893
- to stream Main, to step Merge: 000D83
- to stream PascalFrancis, to step Corpus: 000087
- to stream PascalFrancis, to step Curation: 000004
- to stream PascalFrancis, to step Checkpoint: 000088
- to stream Main, to step Merge: 000E88
- to stream PubMed, to step Corpus: 000343
- to stream PubMed, to step Curation: 000343
- to stream PubMed, to step Checkpoint: 000343
- to stream Ncbi, to step Merge: 000022
- to stream Ncbi, to step Curation: 000022
- to stream Ncbi, to step Checkpoint: 000022
- to stream Main, to step Merge: 000D75
- to stream Main, to step Curation: 000D27
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>ELISA test for rabies antibody titration in orally vaccinated foxes sampled in the fields</title><author><name sortKey="Cliquet, F" sort="Cliquet, F" uniqKey="Cliquet F" first="F" last="Cliquet">F. Cliquet</name></author><author><name sortKey="Sagne, L" sort="Sagne, L" uniqKey="Sagne L" first="L" last="Sagné">L. Sagné</name></author><author><name sortKey="Schereffer, J L" sort="Schereffer, J L" uniqKey="Schereffer J" first="J. L" last="Schereffer">J. L Schereffer</name></author><author><name sortKey="Aubert, M F A" sort="Aubert, M F A" uniqKey="Aubert M" first="M. F. A" last="Aubert">M. F. A Aubert</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:5ABC181646F12B05B459FF73A99003382D75FD2D</idno><date when="2000" year="2000">2000</date><idno type="doi">10.1016/S0264-410X(00)00127-4</idno><idno type="url">https://api.istex.fr/document/5ABC181646F12B05B459FF73A99003382D75FD2D/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000C21</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000C21</idno><idno type="wicri:Area/Istex/Curation">000C20</idno><idno type="wicri:Area/Istex/Checkpoint">000893</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000893</idno><idno type="wicri:doubleKey">0264-410X:2000:Cliquet F:elisa:test:for</idno><idno type="wicri:Area/Main/Merge">000D83</idno><idno type="wicri:source">INIST</idno><idno type="RBID">Pascal:00-0425944</idno><idno type="wicri:Area/PascalFrancis/Corpus">000087</idno><idno type="wicri:Area/PascalFrancis/Curation">000004</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000088</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000088</idno><idno type="wicri:doubleKey">0264-410X:2000:Cliquet F:elisa:test:for</idno><idno type="wicri:Area/Main/Merge">000E88</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:10869772</idno><idno type="wicri:Area/PubMed/Corpus">000343</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000343</idno><idno type="wicri:Area/PubMed/Curation">000343</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000343</idno><idno type="wicri:Area/PubMed/Checkpoint">000343</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000343</idno><idno type="wicri:Area/Ncbi/Merge">000022</idno><idno type="wicri:Area/Ncbi/Curation">000022</idno><idno type="wicri:Area/Ncbi/Checkpoint">000022</idno><idno type="wicri:doubleKey">0264-410X:2000:Cliquet F:elisa:test:for</idno><idno type="wicri:Area/Main/Merge">000D75</idno><idno type="wicri:Area/Main/Curation">000D27</idno><idno type="wicri:Area/Main/Exploration">000D27</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">ELISA test for rabies antibody titration in orally vaccinated foxes sampled in the fields</title><author><name sortKey="Cliquet, F" sort="Cliquet, F" uniqKey="Cliquet F" first="F" last="Cliquet">F. Cliquet</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>AFSSA Nancy, Laboratoire d’études sur la rage et la pathologie des animaux sauvages, B.P. 9, 54220 Malzéville</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Malzéville</settlement></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">France</country></affiliation></author><author><name sortKey="Sagne, L" sort="Sagne, L" uniqKey="Sagne L" first="L" last="Sagné">L. Sagné</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>AFSSA Nancy, Laboratoire d’études sur la rage et la pathologie des animaux sauvages, B.P. 9, 54220 Malzéville</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Malzéville</settlement></placeName></affiliation></author><author><name sortKey="Schereffer, J L" sort="Schereffer, J L" uniqKey="Schereffer J" first="J. L" last="Schereffer">J. L Schereffer</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>AFSSA Nancy, Laboratoire d’études sur la rage et la pathologie des animaux sauvages, B.P. 9, 54220 Malzéville</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Malzéville</settlement></placeName></affiliation></author><author><name sortKey="Aubert, M F A" sort="Aubert, M F A" uniqKey="Aubert M" first="M. F. A" last="Aubert">M. F. A Aubert</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>AFSSA Nancy, Laboratoire d’études sur la rage et la pathologie des animaux sauvages, B.P. 9, 54220 Malzéville</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Malzéville</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Vaccine</title><title level="j" type="abbrev">JVAC</title><idno type="ISSN">0264-410X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">18</biblScope><biblScope unit="issue">28</biblScope><biblScope unit="page" from="3272">3272</biblScope><biblScope unit="page" to="3279">3279</biblScope></imprint><idno type="ISSN">0264-410X</idno></series><idno type="istex">5ABC181646F12B05B459FF73A99003382D75FD2D</idno><idno type="DOI">10.1016/S0264-410X(00)00127-4</idno><idno type="PII">S0264-410X(00)00127-4</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0264-410X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Administration, Oral</term><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Antibody</term><term>ELISA assay</term><term>Enzyme immunoassay</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Fluorescent Antibody Technique</term><term>Foxes (immunology)</term><term>Neutralization Tests</term><term>Rabies</term><term>Rabies Vaccines (immunology)</term><term>Rabies virus</term><term>Sanitary surveillance</term><term>Serology</term><term>Vaccination</term><term>Vaccines, Attenuated (immunology)</term><term>Vaccines, Synthetic (immunology)</term><term>Vulpes vulpes</term><term>Wild animal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Foxes</term><term>Rabies Vaccines</term><term>Vaccines, Attenuated</term><term>Vaccines, Synthetic</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Administration, Oral</term><term>Animals</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Fluorescent Antibody Technique</term><term>Neutralization Tests</term><term>Vaccination</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Animal sauvage</term><term>Anticorps</term><term>Méthode immunoenzymatique</term><term>Rage</term><term>Surveillance sanitaire</term><term>Sérologie</term><term>Technique ELISA</term><term>Vaccination</term><term>Virus rage</term><term>Vulpes vulpes</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Rage</term><term>Vaccination</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The assessment of the efficacy of rabies oral vaccination campaigns requires the titration of specific antibodies in the target species. Unfortunately, in Continental Europe, most fox serum samples are in fact “body fluids” taken from cadavers and the lack of a validated titration method for these poor quality sera made it impossible to survey and compare the efficacy of various oral vaccination protocols used by the different European teams. By using ready to use microplates sensitised with rabies virus glycoprotein purchased from a manufacturer and applying a simple and rapid ELISA technique on field fox sera, we obtained antibody quantitation highly correlated with seroneutralising antibody titres measured with a seroneutralisation test on cell culture. We obtained, with fox sera sampled in the same area, the same distribution of high, medium and low titres within all categories of serum quality (from high to very poor quality) and therefore conclude that this ELISA test allows a reliable titration even with highly contaminated body fluids. This test was shown to be equally capable of detecting rabies antibodies in serum samples taken from foxes vaccinated with an highly attenuated rabies virus (the SAG2 double mutant of the Street Alabama Dufferin strain) or with the VRG, the Vaccinia recombinant glycoprotein. Additionally, a strong correlation was demonstrated between titres given by this ELISA (or by the seroneutralisation test) and protection against challenge of foxes orally vaccinated with SAG2 vaccine baits. In view of this validation, this simple and reliable test is proposed for sero-surveying foxes following rabies oral vaccination campaigns.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Grand Est</li><li>Lorraine (région)</li></region><settlement><li>Malzéville</li></settlement></list><tree><country name="France"><region name="Grand Est"><name sortKey="Cliquet, F" sort="Cliquet, F" uniqKey="Cliquet F" first="F" last="Cliquet">F. Cliquet</name></region><name sortKey="Aubert, M F A" sort="Aubert, M F A" uniqKey="Aubert M" first="M. F. A" last="Aubert">M. F. A Aubert</name><name sortKey="Cliquet, F" sort="Cliquet, F" uniqKey="Cliquet F" first="F" last="Cliquet">F. Cliquet</name><name sortKey="Sagne, L" sort="Sagne, L" uniqKey="Sagne L" first="L" last="Sagné">L. Sagné</name><name sortKey="Schereffer, J L" sort="Schereffer, J L" uniqKey="Schereffer J" first="J. L" last="Schereffer">J. L Schereffer</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/RenardV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D27 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000D27 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= RenardV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:5ABC181646F12B05B459FF73A99003382D75FD2D
|texte= ELISA test for rabies antibody titration in orally vaccinated foxes sampled in the fields
}}
| This area was generated with Dilib version V0.6.27. |  |